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Biotechnology Information homology calculation algorithm ncbi blast
Homology Calculation Algorithm Ncbi Blast, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Twelve novel SNPs of the goat POU1F1 gene and their associations with cashmere traits
Article Snippet: As a member of the POU-domain family, pituitary-specific transcription factor 1 (POU1F1) is responsible for a phenotype of combined pituitary hormone deficiency (CPHD) involving growth hormone (GH), prolactin (PRL) and thyrotropin subunit (TSH ).. Owing to PRL concentrations associated with wool growth and wool traits, the POU1F1 gene is a potential candidate gene for cashmere traits selection and breeding through marker-assisted selection (MAS).. We used PCR–SSCP and DNA sequencing to screen for genetic variations of the unreported exons 1–5 and 5′-UTR within POU1F1 gene in 452 Inner Mongolian white cashmere goats.

Article Title: Influences of melatonin treatment, melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) gene polymorphisms on first conception in Sarda ewe lambs
Article Snippet: In order to investigate if the melatonin receptor 1A (MTNR1A) and kisspeptin (KiSS-1) genes influence the reproductive response to melatonin treatment, 510 Sarda ewe lambs were divided into groups C (control) andM; GroupM received one melatonin implant (18mg).. After 35 days rams were introduced for 40 days and subsequent lambing dates and number of newborns were recorded.. The MTNR1A gene Exon II and KiSS-1 gene Exon I were amplified and genotyped by restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism analysis.

Article Title: Unveiling characteristics of a bioelectrochemical system with polarity reversion for simultaneous azo dye treatment and bioelectricity generation.
Article Snippet: A novel bioelectrochemical system (BES) operated with polarity reversion was explored for simultaneous anaerobic/aerobic treatment of azo dye and production of bioelectricity under extremely low buffer.. The Congo red was first decolorized in anode, with completed color removal in 35 h. The resultant decolorization intermediates were then mineralized after the anode reversed to aerobic biocathode, evidenced by 55 % chemical oxygen demand (COD) removal in 200 h. The mineralization efficiency was further increased to 70 % when the period of the half-cycle was prolonged to 375 h. Meanwhile, the BES produced a continuous stable positive/negative alternate voltage output under 5 mM phosphate buffer because of the self-neutralization of the accumulated protons and hydroxyl ions in electrolyte.. The electrode performance was significantly improved, which was indicated by alleviated electrode polarization, due to in situ use of accumulated protons and hydroxyl ions and enhanced electron transfer in the presence of Congo red and its degradation intermediates, which resulted in 1.05-fold increases in maximum power density (67.5 vs. 32.9 mW/m).

Article Title: Polymorphisms in the Growth Hormone Gene and Their Association with Pre-pullet Body Weight in Kuttanad Ducks of Kerala, India
Article Snippet: The NCBI (National Centre for Biotechnology Information) Blast algorithm was used to search the NCBI GenBank database (http:/ www.ncbi.nlm.nih.gov/) for homologous sequences.

Article Title: Identification of novel SNPs in the Sarda breed goats POU1F1 gene and their association with milk productive performance.
Article Snippet: The aim of the study was to detect polymorphism in the POU1F1 gene in Sarda breed goat, as well as to establish if SNPs could be associated with milk productive traits.. The research was conducted on 129 Sarda breed goats from 4 to 5 years old, multiparous, lactating and in their third to fifth lactation.. We report nine exonic and seven non-coding regions SNPs within the Sarda goat POU1F1 gene, namely, Ex 1 61 G[C; Ex 1 108 G[A; Ex 3 C[T; Ex 3 92 C[T; Ex 4 110 A[G; Ex 5 34 G[A resulting in Arg213Lys change; IVS4 641 G[A, IVS4 643 A[C, IVS4 659 G[A, IVS4 677 A[C, IVS4 G699Del, IVS4 709 C[G, Ex 6 17 G[A resulting in Arg228Ser change, Ex 6 58 G[T, Ex 6 172 T[C, 30UTR 110 T[C. A statistically significant association was found between genotype TT, in position 17 of the exon 6 (3.1 % of frequency), and increased milk yield (P \ 0.01) while genotype GT (25.6 % of frequency) was associated with a higher fat content.

Article Title: The life-cycle of the avian haemosporidian parasite Haemoproteus majoris, with emphasis on the exoerythrocytic and sporogonic development
Article Snippet: Additionally, several lineages that are most similar to the known lineages of this parasite were selected using the National Center for Biotechnology Information (NCBI) BLAST algorithm.

Sequencing:

Article Title: Glutaredoxin 1 from big-belly seahorse (Hippocampus abdominalis): Molecular, transcriptional, and functional evidence in teleost immune responses.
Article Snippet: Accepted Manuscript Glutaredoxin 1 from big-belly seahorse (Hippocampus abdominalis): Molecular, transcriptional, and functional evidence in teleost immune responses W.K.M.. Omeka, D.S.. Liyanage, Thanthrige Thiunuwan Priyathilaka, G.I.

Polymerase Chain Reaction:

Article Title: Replacement of Trypsin by Proteases for Medical Applications
Article Snippet: .. The PCR products were sequenced and compared with other sequences in the GenBank using the National Center for Biotechnology Information (NCBI) BLAST algorithm. .. The bacterial occultation cultures in 4 mL BHI broth (Merck, Germany) were incubated at 25°C for 48 hours.



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